Maximum likelihood species tree of 22 Saccharomycotina species. The tree was reconstructed based on the analysis of a concatenated alignment of one-to-one orthologs of 603 widespread genes. Species names in red and with an asterisk indicate human fungal pathogens. Underlined species names correspond to the newly sequenced Nakaseomyces species. Important evolutionary events such as the Whole Genome Duplication (WGD) or the genetic code transition in the Candida clade (CTG) are marked on the tree. All aLRT-based supports were maximal and a single node with a bootstrap support below 100% is indicated. This topology is also the most parsimonious in terms of inferred duplications in 4,965 individual gene phylogenies, as assessed by a Gene Tree Parsimony approach implemented in duptree .