Methylation and expression of BGIBMGA000155. (A) Comparison of MethylC-Seq data of the BGIMBGA000155 DMC with the 454 sequencing data of traditional bisulfite PCR (BS-PCR) sequencing. Detail sample and method information is the same as in Figure 3(A). (B) Location of the DMC cluster in genic regions. The gene model is at bottom, where blocks indicate exons (red for constitutive exons and blue for alternative exons, respectively) and the black lines between each two blocks indicate introns. Dome, domesticated silkworms, Wild, wild silkworms. (C) BGIBMGA000155 expression level changes in silk gland of each domesticated (Dazao, Js, C108, L10, HY, ZZ and 872) individual against wild silkworms (Wsh, Wyn, CW, XW, Wzj, Wjs and Wgs), estimated by quantitative real-time PCR, indicating that this gene is generally up-expressed in the domesticated silkworms. Relative expression ratios of each domesticated to wild silkworms are normalized by logarithmic transformation. Plots show the average relative expression ratios and error bars show the standard errors. (D) Expression of BGIBMGA000155 in different tissues of Dazao examined by microarray analysis, indicating this gene has a higher expression level in silk gland. Plots show the average intensities and error bars show the standard errors.