Figure 2From: Combining in silico prediction and ribosome profiling in a genome-wide search for novel putatively coding sORFsThe combined approach identifies many putatively functional sORFs in intergenic regions. (A) Visual representation of the intergenic sORF located on the forward strand of chromosome 11 (69,794,326-69,794,388) based on data from the H2G2 genome browser. (B) DNA multiple alignments for the intergenic sORF presented in Figure 2A and based on the 8 species under investigation from the UCSC mm9 multi-species alignment. (C) Visual representation of the overlap between intergenic sORFs with ribosomal profiling evidence and the classified test subjects. True coding sORFs are depicted in green and true non-coding in red (see Additional file 1: Figure S2), black dots represent the intergenic sORFs. Classification and presentation are based on the coding probability scores from the 2 SVMs used during the analysis (See Methods). (D) Visual representation of the intergenic sORF located on the reverse strand of chromosome X (71,212,050-71,212,082) based on data from the H2G2 genome browser. The sORF is located approximately 400 bp upstream of a known protein-coding gene (Hcfc1).Back to article page