Genome-wide identification of soybean microRNAs and their targets reveals their organ-specificity and responses to phosphate starvation

Table 1 Statistics of four small RNA libraries from soybean

	Library name^a
	Leaf+Pi (HPL)	Leaf-Pi (LPL)	Root+Pi (HPR)	Root-Pi (LPR)
Total reads	22925183	26658394	21456069	28635677
High quality reads	22643245(100%)	26377550(100%)	20957370(100%)	27944092(100%)
Reads smaller than 18nt	126772(0.56%)	68893(0.26%)	347577(1.66%)	800247(2.86%)
Clean reads	22483373(99.29%)	26268210(99.59%)	20564547(98.13%)	27083979(96.92%)
Total small RNA reads mapping to genome^b	18248364/22483373 (81.16%)	22176679/26268210 (84.42%)	20591098/27083979 (76.03%)	15573680/20564547 (75.73%)
Unique small RNA reads mapping to genome^b	2396442/2901995 (82.58%)	3301579/3951946 (83.54%)	2360847/4071174 (57.99%)	2583592/4641188 (55.67%)

^aFour small RNA libraries were constructed. Total RNA were extracted from soybean leaves and roots grown in +Pi or -Pi nutrient solutions.
Accordingly the small RNA libraries were named leaf+Pi (HPL), leaf-Pi (LPL), root+Pi (HPR), and root-Pi (LPR).
^bTotal small RNA reads and unique small RNA reads were mapped to the Glycine max genome v1.0 sequences available in Phytozome (http://www.phytozome.org).

ISSN: 1471-2164