Correction and insertion of annotated genes. (A) Correction of start codons. (Upper panel) Transcriptional activity of pooled RNA-Seq data. The grey arrow displays the coordinates of the ribose operon repressor RbsR (BLi03840) according to Veith et al. . Based on the transcriptional data, the start codon has been reassigned 57 bp downstream of the former position (orange arrow). (Lower panel) The new start codon is marked in orange and the transcription start site in blue. The location of patterns of a ribosomal binding site and -10 and -35 regions of the rbsR- regulating σA upstream of the gene provide additional confirmation to the new annotation. (B) Insertion of new genes. (Upper panel) Transcriptional activities (sample L-I) of BLi03658 (black arrow) and indep RNA BLi_r2780 (green arrow). The previously not detected [1, 9] protein gene BLi05038 (orange arrow) was annotated as BsrG-like peptide (see also chapter Comparative transcriptomics). (Lower panel) The start codon of the new gene is marked in orange, and the transcription start site in green. The location of patterns of a ribosomal binding site and a σA -10 and -35 promoter region provide additional confirmation of the new annotation.