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Table 1 Molecular analysis of transgenic embryos using Pv010 and nptII primers

From: Stacking resistance to crown gall and nematodes in walnut rootstocks

Walnut genotypes and vector combinations

Samples PCR tested using nptII and Pv010 primers (no.)

Percentage co-transformed or single-vector transformed

J1 - 1:3 CG:Pv010

77

5/77 (6.49%)1

J1 - 1:1 CG:Pv010

23

4/23 (17.39%)1

RR4 - 1:3 CG:Pv010

1

1/1 (100%)1,2

RR4 - 1:1 CG:Pv010

7

5/7 (71.42%)1

J1- Pv 010

25

18/25 (72%)3

J1- Pv 194

25

23/25 (92%)4

  1. 1Co-transformed percentage.
  2. 2The only transgenic line that survived was positive for both Pv10 and nptII.
  3. 3Single-vector transformed percentage. These samples only tested with Pv010 primers.
  4. 4Single-vector transformed percentage. These samples only tested with Pv194 primers.
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BMC Genomics

ISSN: 1471-2164

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