Skip to main content

Table 1 Molecular analysis of transgenic embryos using Pv010 and nptII primers

From: Stacking resistance to crown gall and nematodes in walnut rootstocks

Walnut genotypes and vector combinations Samples PCR tested using nptII and Pv010 primers (no.) Percentage co-transformed or single-vector transformed
J1 - 1:3 CG:Pv010 77 5/77 (6.49%)1
J1 - 1:1 CG:Pv010 23 4/23 (17.39%)1
RR4 - 1:3 CG:Pv010 1 1/1 (100%)1,2
RR4 - 1:1 CG:Pv010 7 5/7 (71.42%)1
J1- Pv 010 25 18/25 (72%)3
J1- Pv 194 25 23/25 (92%)4
  1. 1Co-transformed percentage.
  2. 2The only transgenic line that survived was positive for both Pv10 and nptII.
  3. 3Single-vector transformed percentage. These samples only tested with Pv010 primers.
  4. 4Single-vector transformed percentage. These samples only tested with Pv194 primers.