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Table 2 Genotypes of transformed J1 and RR4 walnut rootstocks determined by PCR

From: Stacking resistance to crown gall and nematodes in walnut rootstocks

Walnut genotypes Vector combinations Embryo ID no. GUS nptII Pv010 Pv194 iaaM/ipt rol A rol B rol C
J1 1:3 32-2-2 + + + _ + + + +
33-3-1 + + + _ + + + +
66-1-2 + + + _ + + + +
68-3-1 + + + _ + + + +
73-2-1 + + + _ + + + +
1:1 2-2-1 + + + _ + + + +
6-2-1 + + + _ + + + +
21-2-1 + + + _ + + + +
23-3-2 + + + _ + + + +
RR4 1:3 38-1-1 + + + _ + + + +
1:1 7 -2-1 + + + _ + + + +
8 -1-1 + + + _ + + + +
23-1-1 + + + _ + + + +
44-1-1 + + + _ + + + +
73-1-2 + + + _ + + + +
J11 11 2-1-1 + + + _ _ _ _ _
15-3-1 + + + _ _ _ _ _
24-2-2 + + + _ _ _ _ _
29-3-1 + + + _ _ _ _ _
30-2-2 + + + _ _ _ _ _
11 8-1-1 + + _ + _ _ _ _
13-3-2 + + _ + _ _ _ _
15-3-1 + + _ + _ _ _ _
24-2-1 + + _ + _ _ _ _
  1. 1One vector (A. tumefaciens carrying Pv010 gene or Pv194) used for transformation.