Stacking resistance to crown gall and nematodes in walnut rootstocks

BMC Genomics

Table 2 Genotypes of transformed J1 and RR4 walnut rootstocks determined by PCR

Walnut genotypes	Vector combinations	Embryo ID no.	GUS	nptII	Pv010	Pv194	iaaM/ipt	rol A	rol B	rol C
J1	1:3	32-2-2	+	+	+	_	+	+	+	+
		33-3-1	+	+	+	_	+	+	+	+
		66-1-2	+	+	+	_	+	+	+	+
		68-3-1	+	+	+	_	+	+	+	+
		73-2-1	+	+	+	_	+	+	+	+
	1:1	2-2-1	+	+	+	_	+	+	+	+
		6-2-1	+	+	+	_	+	+	+	+
		21-2-1	+	+	+	_	+	+	+	+
		23-3-2	+	+	+	_	+	+	+	+
RR4	1:3	38-1-1	+	+	+	_	+	+	+	+
	1:1	7 -2-1	+	+	+	_	+	+	+	+
		8 -1-1	+	+	+	_	+	+	+	+
		23-1-1	+	+	+	_	+	+	+	+
		44-1-1	+	+	+	_	+	+	+	+
		73-1-2	+	+	+	_	+	+	+	+
J1¹	1¹	2-1-1	+	+	+	_	_	_	_	_
		15-3-1	+	+	+	_	_	_	_	_
		24-2-2	+	+	+	_	_	_	_	_
		29-3-1	+	+	+	_	_	_	_	_
		30-2-2	+	+	+	_	_	_	_	_
	1¹	8-1-1	+	+	_	+	_	_	_	_
		13-3-2	+	+	_	+	_	_	_	_
		15-3-1	+	+	_	+	_	_	_	_
		24-2-1	+	+	_	+	_	_	_	_

¹One vector (A. tumefaciens carrying Pv010 gene or Pv194) used for transformation.

ISSN: 1471-2164