Dot plots of hybrid and PacBio-only assemblies. BL21(DE3) was assembled with varying conditions as described. The best assembly for each combination of technology and PacBio coverage was determined using Amosvalidate. Assemblies were scaffolded with AHA, gap-filled with PBJelly and finally polished with Quiver. The resulting assemblies were aligned to the reference genome and dot plots generated with MUMmer.