InsertionMapper: a pipeline tool for the identification of targeted sequences from multidimensional high throughput sequencing data

Table 1 Dsg transposition in maize genome

Well	Chr	Location	Dsg insertion sequence	Gene model	Dsg exon insertion	Gene description
20G08	1	275382862	GTAGCATC…	GRMZM2G010034	2/6	Uncharacterized protein
20H04	2	40009418	CAGATGAC…	GRMZM2G327295	2/4	Putative copper transport protein
20H07	10	10196376	CAAGTGAA…	GRMZM2G046284	2/2	Fructose bisphosphate aldolase
20H11	9	154438893	GTCGCCGA…	GRMZM2G092718	3/3	Putative copper transport protein
21B12	9	152260129	GGCGTGCA…	GRMZM2G438840	2/2	Putative leucine rich-repeat transmemberane protein kinase
21B12	9	102784988	GTCTGAAC…	GRMZM2G006790	1/4	Putative calcium binding protein CML21
21C05	7	164656311	TCTGCGGC…	GRMZM2G082205	2/22	Putative protein
21D01	9	154438893	GTCGCCGA…	GRMZM2G092718	3/3	Putative copper transport protein
21D03	2	40150103	GTGCCATT…	GRMZM2G050435	3/5	Hypothetical protein
21D06	7	11443820	CTTGTATA…	GRMZM2G401308	5/5	Histone H1-3

10 Dsg s from plate 20 and plate 21 are shown with their 3-D coordinates in sample pools (Well), chromosome (Chr) locations on the maize B73 genome (Location), 8-bp target site duplication (TSD) of host junction sequences (Dsg insertion sequence), sequences within ±1kb to the insertion site (Gene model), the exon where Dsg inserted relative to the total number of exons in that gene (Dsg exon insertion) and the gene description. A full list of sequence-indexed Dsg s can be viewed at our project website http://acdsinsertions.org/showt/dsg.

ISSN: 1471-2164