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Table 1 Predicted TF sites in the PRE

From: PuF, an antimetastatic and developmental signaling protein, interacts with the Alzheimer’s amyloid-β precursor protein via a tissue-specific proximal regulatory element (PRE)

Factor

Sequence within PREa

MW (kDa)c

AP2

GGGGTGGGCCG

32, 40, 47, 48, 49, 50, 51, 52

BTEB1e

GAGCGG

 
 

GGCCGG

 

BTEB2e

GAGCGG

25

 

GGCCGG

 

GATA1

GGGTGGGC

43, 51

 

CCGGATCAGc

 

GATA2

CCGGATCAGc

50

Msx1e

GAGCGG

31

 

GGCCGG

 

Pax4α

GGGGTGGGCCGG

38

PuF

GGGTGGG

17

R

GTGCCGAGCGGGGTGGGC

67

SP1b,e

GGGTGG

81, 95, 105

 

TGGGCCGGATCAGctg

 
 

GAGCGG

 
 

GGCCGG

 

TCERG1

CGAGTG

122, 124d

 

CGAGCG

 

USF1

GGATCAGctgactc

34, 43, 55

USF2

GGATCAGctgactc

37, 44

  1. aAll sequences presented in the same orientation as the PRE. Partial sites are also listed, with bases that extend past the PRE indicated by dashes.
  2. bTwo distinct SP1 sites were identified in the wildtype PRE sequence in addition to the GC Box.
  3. cUnless otherwise indicated, MW are for isoforms listed in the TransFac database [42].
  4. dMW is calculated from predicted genomic amino acid sequence.
  5. eOne of several potential GC Box-binding factors.