Table 8 Oligomers for EMSA of PRE-derived mutants
Oligomer | Forward (F) 5′ to 3′a | Reverse (R) 5′ to 3′ |
|---|---|---|
PRE (WT) | 5′-TCGAGTGCCGAGCGGGGTGGGCCGGATCAG-3′ | 5′-CTGATCCGGCCCACCCCGCTCGGCACTCGA-3′ |
M1 | 5′-TCGAGTGCCGAGCGGGGTGGTA CC GATCAG-3′ | 5′-CTGATCG GTA CCACCCCGCTCGGCACTCGA-3′ |
M4 | 5′-TCGAGTGCCGAGCGGGGTGGGCCT GTA CAG-3′ | 5′-CTGTA CA GGCCCACCCCGCTCGGCACTCGA-3′ |
M2 | 5′-TCGAGTGCCGAGCGCT GTGGGCCGGATCAG-3′ | 5′-CTGATCCGGCCCACAG CGCTCGGCACTCGA-3′ |
M3 | 5′-TCGAGTCG CGAGCGGGGTGGGCCGGATCAG-3′ | 5′-CTGATCCGGCCCACCCCGCTCGCG ACTCGA-3′ |
M4 | 5′-TCC ATG GCCGAGCGGGGTGGGCCGGATCAG-3′ | 5′-CTGATCCGGCCCACCCCGCTCGGCCA TG GA-3′ |
M6 | 5′-TCGAGTGCCGAT CGGGGTGGGCCGGATCAG-3′ | 5′-CTGATCCGGCCCACCCCGA TCGGCACTCGA-3′ |
M7 | 5′-TCGAGTC CG GAGCGGGGTGGGCCGGATCAG-3′ | 5′-CTGATCCGGCCCACCCCGCTC CGG ACTCGA-3′ |