Table 9 Oligomers for site-directed mutagenesis of the pβXbB PRE/CAT fusion plasmid
Oligomer | Forward (F) 5′ to 3′ | Reverse (R) 5′ to 3′ | REa |
|---|---|---|---|
M1m-F | 5′-CCGAGCGGGGTGGTA CC GATCAGCTGACTTGCC-3′ | 5′-GGCAAGTCAGCTGATCG GTA CCACCCCGCTCGG-3′ | Kpn I |
M4m-F | 5′-CGAGCGGGGTGGGCCT GTA CAGCTGACTTGCC-3′ | 5′-GGCAAGTCAGCTGTA CA GGCCCACCCCGCTCG-3′ | Bsr GI |
M2m-F | 5′-TCGAGTGCCGAGCGCT GTGGGCCGGATCAG-3′ | 5′-CTGATCCGGCCCACAG CGCTCGGCACTCGA-3′ | Afe I |
M3m-F | 5′-CTGGATCTCGAGTCG CGAGCGGGGTGGGC-3′ | 5′-GCCCACCCCGCTCGCG ACTCGAGATCCAG-3′ | Nru I |
M4m-F | 5′-CCAGATCTGGATCTCC ATG GCCGAGCGGGGTGGG-3′ | 5′-CCCACCCCGCTCGGCCA TG GAGATCCAGATCTGG-3′ | Nco I |
M6m-F | 5′-CTCGAGTGCCGAT CGGGGTGGGCCG-3′ | 5′-CGGCCCACCCCGA TCGGCACTCGAG-3′ | Pvu I |
M7m-F | 5′-CTGGATCTCGAGTC CG GAGCGGGGTGGGCCG-3′ | 5′-CGGCCCACCCCGCTCC GG ACTCGAGATCCAG-3′ | Bsp EI |