Figure 5

Methylation and expression analyses of AIM1 in macaque placenta. (A) The region homologous to the AIM1 DMR in the macaque exhibited approximately 34% methylation. (B) Sequencing of clones from the heterozygous individual pl36 showed that the expressed allele (G allele) was associated with unmethylated CpGs, while the non-expressed allele (C allele) was partially methylated. (C) AIM1 was monoallelically expressed in the macaque. Arrow depicts the SNP location for each sample. In all four samples, the DNA was heterozygous and the RNA showed monoallelic expression.