Identification of a single reciprocal translocation. (A) Dot plot representation of DNA sequence identity between the S. cerevisiae and S. arboricolus genomes. A single reciprocal translocation is apparent between chromosomes IV and XIII. (B) Cartoon representation of the location of the reciprocal translocation including the flanking features (red: Watson strand ORFs; blue: Crick strand ORFs; green: tRNA genes; yellow: autonomously replication sequence (ARS)[16, 17]) and the primer locations (not to scale) used to confirm the translocation. (C) PCR-based confirmation of the reciprocal translocation. Various primer and template combinations (as indicated) were used to amplify products corresponding to either the S. cerevisiae or the S. arboricolus gene order. In each case the resulting PCR products support the reciprocal translocation identified by the genome assembly.