Proteogenomics approach for novel protein annotation. Examples of novel open reading frames (ORFs) detected by proteogenomics. ORFs (red) were detected by the in-frame expansion of peptide spectra (purple) that uniquely map to un-annotated intergenic regions. The RNA sequencing coverage profile from various sample time points (black histogram) associated with these examples is also indicated. (i) Expression of these novel proteins displaying a dynamic transcriptional profile. (ii) Validation of novel ORF found with Prodigal 2. (iii) A small RNA associated with the initiation codon.