Figure 2

New conopeptide precursors isolated from the venom duct transcriptome of Conus marmoreus and MS/MS coverage. New full-length precursor conopeptides inferred from mRNA data, and detected with ConoSorter simultaneously in the signal, pro-, and mature regions without conflict (isoforms of these sequences can be found in Additional file3: Figure S2). For each known superfamily, the sequence alignment shows the amino acid conservation specifically in the N-terminal signal region (the deeper color the more conserved the amino acids are). Partial sequences in bold characters correspond to the peptide fragments isolated by MS/MS analysis of C. marmoreus milked venom. Numbers listed after conopeptide names indicate the sequence frequency in the input data. DNA Data Bank of Japan (DDBJ) accession numbers of full-length precursors, as well as post-translational modifications (PTMs) calculated by ProteinPilot 4.0 are provided in Additional file4: Table S2.