Figure 1

Workflow for a small RNA-Seq approach in C. glutamicum ATCC 13032. Before cDNA library preparation, the small RNA fraction was split into two samples for creation of two different sequencing libraries. The first sample was treated with a 5′-monophosphate-specific exonuclease to degrade transcripts that are processed or undergoing degradation. The second sample was left untreated and represents the whole of sRNA transcripts within the cell. After cDNA library preparation, both samples were then separately committed to strand-specific high-throughput sequencing.