Table 4 Primers used for sequencing validation of SNPs discovered between ten Brassica napus accessions
From: Identification of genome-wide single nucleotide polymorphisms in allopolyploid crop Brassica napus
Primer_Name | SNP_type | Locus | Forward_primer | Tm(°C) | Reverse primer | Tm(°C) |
|---|---|---|---|---|---|---|
ns001 | A/G | BRscaffold000003-1385327 | CATCAGGGAAATGGAGAGGA | 60 | GTGCACCAGCTCTCAAACAA | 60 |
ns002 | A/G | BRscaffold000027-2243593 | CGGTTTAGGATCCGAGTTGA | 60 | CACGTCGCTACTGCAGCTTA | 60 |
ns003 | A/G | BOscaffold000050-1147091 | CAGTGCTTGGCTCGTGTCTA | 60 | ATTCTGAATTCCGTTGACCG | 60 |
ns004 | G/T | BRscaffold000039-1828478 | TCTGTCGGCTCTGTCATCTG | 60 | TCCGGTTCAGTTTCTGGTTC | 60 |
ns005 | A/G | BOscaffold000131-442418 | GCTTTTGGTGTGGACATCCT | 60 | GAGATCCTGGGTCAACCAAA | 60 |
ns006 | G/C | BOscaffold000197-783005 | CGATCGTCATACTCGGACCT | 60 | TTCCGATTCTGCCTCCTCTA | 60 |
ns007 | G/C | BOscaffold000230-556330 | GCAGCTGATATTGCTGTGGA | 60 | TTGTTTCAATCCGCACAAAG | 60 |
ns008 | A/G | BOscaffold000244-357760 | CGTAACGTTTGGGCTGTTTT | 60 | ATGGTCGGCCATGTTTTTAG | 60 |
ns009 | C/T | BOscaffold000265-92497 | CACTAGCTTCGCATCAACCA | 60 | TGAGGTGTCATCGATAAGCG | 60 |
ns010 | C/T | BRscaffold000130-113927 | TGATCGGGTTGTACACATGG | 60 | AGGACGGCCTTCATTATTCT | 58 |