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Figure 4 | BMC Genomics

Figure 4

From: Loss of WSTF results in spontaneous fluctuations of heterochromatin formation and resolution, combined with substantial changes to gene expression

Figure 4

BAZ1B -/- DAPI-dense blocks are characterized by constitutive heterochromatin markers. (a) Association of endogenous HP1α, HP1β, and HP1γ (green) with DAPI-dense blocks. Nuclei are counterstained with DAPI (blue) and merged images are shown in the right-side panel. (b) Association of exogenous GFP-tagged HP1α, HP1β, and HP1γ (green) with DAPI-dense blocks. (c) Examples of elevated levels of H3K9me3 (green) at DAPI-dense blocks. (d) Western blot showing comparable levels of H3K9me3 in mutant compared to RPE1. Anti-histone H4 is used as a loading control. (e) Example of the association of SMCHD1 (green) with DAPI-dense blocks. (f) Examples showing the lack of association of euchromatin markers H3K4me2 and H3K9Ac with the DAPI-dense blocks.

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BMC Genomics

ISSN: 1471-2164

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