Table 1 Primers and probes used for the RT-PCR assays
Gene | GenBank ID | Forward primers (5′-3′) | Probes (5′-3′) | Reverse primers (5′-3′) |
|---|---|---|---|---|
STAT1 | NM_001012914 | AAGCAAACGTAATCTTCAGGATAAC | (FAM)-CAAGAAGACCCGATACACATGGCAA-(BHQ1) | TTTCTCTCCTCTTTCAGACAGTTG |
ISG12-2 | NM_001001296 | TTCCACTATCCAGTCTATCTCAATG | (FAM)-ACCTGCTCCTGGACCGATGCTTCTT-(BHQ1) | GTGAATCTGTCTGTAAAGGATGAAC |
SOCS3 | NM_204600 | ACTGCGCCCCAGGTGATG | (FAM)-CTCCCGGCAGCAGCACCCC-(BHQ1) | GGGAACTTGCTGTGGGTGAC |
TNFAIP6 | NM_001037837 | GAGGCAGCGAGAAAAATAGGTTTC | (FAM)-CCATCCAGCCAGCAGCACACAC-(BHQ1) | GCTTTTACTATGGGGTAACCAACTC |
IRG1 | NM_001030821 | TCCGAGATGTGGGCAAAGAC | (FAM)-TCGCTTGCTTCTCTGAATGACCACA-(BHQ1) | CCTACTCCAAGGGTATCCAGAATC |
SPP1 | NM_204535 | CCAGAACAGCCGGACTTTC | (FAM)-TGACATTCCTAGCAAGAGCCAAGAG-(BHQ1) | TGGAATCATTGTCATCATCATCATC |
18S | FM165414 | GGTTGCAAAGCTGAAACTTAAAGG | (FAM)-ACTCCTGGTGGTGCCCTTCCGTCAA-(BHQ1) | TGAGGTTTCCCGTGTTGAGTC |
IFIT5 | XM_421662 | AAAAGAAGGCAAATCATGAGTACC | (FAM)-AATTCCTTGAAGAACTCCCTGCTGC-(BHQ1) | TGATCCTCTATTGATTCTTCCAGAC |
MX1 | NM_204609 | AGAAAGCAATAAGAAAAGCCCAAG | (FAM)-AATGCTACCATTAGTGCCAGCCAC-(BHQ1) | ACCAGATTTCAAGGGAAATTAGTTC |
OASL | NM_205041 | AGCACTGGTACAAGGAGATGTTG | (FAM)-CTGAAGTCCTCCCTGCCTGTGCCCT-(BHQ1) | CCAAGCAGCTCCAGCACAG |
RSAD2 | XM_426208 | CTTAAGGAGGCGGGAATGGAG | (FAM)-TTGCTCACAATGCTGACGCTTGGC-(BHQ1) | TTGAACCACCGTTCCCTGATC |