Figure 1

Kinetics of selected metabolites/hormones and expression of liver genes during a 48 hour fasting period. (A) and (B) Serum parameters and liver mRNA levels were measured in 10–12 weeks old fasted and ad-libitum fed male C57Bl/6 J mice at several time points (each n = 5). Shading indicates light or dark cycle. Relative expression values from qPCR measurements were normalized to 36b4 expression and the values for time point 0 were set to 1. Significance was determined with a 2-way ANOVA followed by a Bonferroni posttest to determine significance for the single time points (*p < 0.05; **p < 0.01; ***p < 0.001).