Expression analysis of putative long non-coding RNAs (NGM-vvo). (a) Boxplot to show log transformed expression (RPKM, PolyA+) across six developmental stages in the NGM-vvo subset. (b) Density graph to compare stage-9 expression (RPKM, PolyA+) between all gene models (GM) and NGM-vvo subset. (c) Heat map to show unsupervised hierarchical clustering of expression (RPKM) of polyA+ and RZ data across embryogenesis. Colorscale represents deviation from mean expression calculated row-wise. (d) Density plot to compare distribution of log10 transformed conservation score (phastCons analysis, see Materials and methods) between random genomic sequences (Genomic Seq), Xenbase extracted X.tropicalis mRNAs (X.trop mRNA) and NGM-vvo subset. PolyA+(RNA harvested with double polyA+ selection), RZ (ribosomal rRNA depleted-total RNA).