Figure 3

Validation of ten differentially expressed genes using quantitative PCR on independently generated biological replicate sample sets as templates. Transcripts were randomly selected for validation and their expression considered significantly regulated (*) if p < .05 using a 1-way ANOVA with Bonferroni post-test analysis (Graphpad Prism v.5.0a). Expression values as cycle thresholds were normalized to those of a house keeping gene, BBOV_III004820, thus, the final data are represented as cycle threshold ratio (CT ratio) where the lower the ratio value, the higher the expression. III 010740, gene encoding for 1-deoxy-D-xylose-5-phosphate reductoisomerase; III006460 and III006500, genes encoding for spherical body protein 2 truncated copies 7 and 9 respectively; II007790, gene encoding for a hypothetical protein; III006070, III001500, I004520 and III003100, genes encoding for variant erythrocyte surface (ves) α subunits; III006080 and IV001490, genes encoding for ves β subunits and III002360, gene encoding for a putative membrane protein.