Figure 5

Gene expression in B. oleracea phloem-enriched strands control tissue. Semi-quantitative RT-PCR analysis of gene expression in B. oleracea phloem-enriched strands (P) and non-phloem containing control tissue (NP). A. Controls: four genes encoding housekeeping genes with expression in both phloem and non-phloem tissues and three phloem-specific genes encoding sucrose synthase 6, SEOR1, and SEOR2. B. The expression of genes encoding proteins previously identified as phloem-specific that were identified in the B. oleracea proteome. C. The expression of genes encoding proteins identified in the B. oleracea proteome that were not previously identified as phloem-specific proteins. *Myrosinase 1 primers also amplified myrosinase 2 (At4G26000).