Table 1 Classification of identified proteins in honeybee venom manually extracted from venom gland and electrical stimulation by gel-based and gel-free techniques

gi|5627

Phospholipase A-2 (Api m 1)

Bee venom toxins

Y

2, 57

gi|58585182

Hyaluronidase precursor (Api m 2)

Y

2, 57

gi|66821891

Venom allergen acid phosphatase (Api m 3)

N

2

gi|28201825

Melittin (Api m 4)

Y

2, 57

gi|187281543

Venom dipeptidyl peptidase IV precursor (Api m 5)

Y

2, 57

gi|94400907

Allergen Api m 6 precursor (Api m 6)

Y

2, 57

gi|187281550

Venom carboxylesterase-6 precursor (Api m 8)

Y

2, 57

gi|60115688

Icarapin-like precursor (Api m 10)

Y

2, 57

gi|67010041

Major royal jelly protein 9 precursor (Api m 11)

Y

2, 57

gi|58585070

Major royal jelly protein 8 precursor (Api m 11)

Y

2, 57

gi|58585166

Apamin preproprotein

Y

2, 57

gi|223850

Secapin

N

5,38

gi|110758297

Phospholipase A2-like

Y

2, 57

gi|1708948

Mast cell degranulating peptide

Y

2, 57

gi|58585116

Venom serine protease 34 precursor (Api m 7)

Y

2, 57

gi|226533687

Venom serine protease 34 precursor Venom serine carboxypeptidase precursor (Api m 9)

Y

2, 57

gi|58585146

Arginine kinase

N

36

gi|58585104

Vitellogenin (Api m 12)

Y

57

gi|380020933

Glutathione S-transferase-like isoform 1 (GstS1)

Antioxidant systems

N

57

gi|58585086

Transferrin 1 precursor (Tsf1)

Y

57

gi|110755367

Toll-like receptor 13-like isoform 1 (TLRs)

Y

57

gi|66514614

Chitinase-like protein Idgf4-like

Y

57

gi|283436152

Peroxiredoxin-like protein (Prx)

N

57

gi|295849268

Superoxide dismutase 1 (Sod1)

N

57

gi|22982210

Heat shock protein cognate 4 (Hsc)

N

57

gi|149939403

Hexamerin

N

57

gi|328790510

DnaJ homolog subfamily B member 11-like

Protein folding and molecular transporters

Y

57

gi|335892796

Peptidyl-prolyl cis-trans isomerase B precursor

Y

57

gi|328782499

Proactivator polypeptide isoform 1

Y

57

gi|328780884

Apolipophorins isoform 1

N

57

gi|328780886

Apolipophorins-like

Y

57

gi|110749558

Histone H2B.3-like

N

57

gi|66535784

Odorant binding protein 21 precursor (Obp 21)

Y

57

gi|66515272

V-type proton ATPase catalytic subunit A-like (V-ATPase subunit A)

Carbohydrate and energy metabolism

N

57

gi|328785025

ATP synthase subunit beta, mitochondrial (ATPsyn-beta)

N

57

gi|328776580

Enolase-like (Eno)

N

57

gi|66525576

Aldose reductase-like (AR)

N

57

gi|328780312

Alcohol dehydrogenase [NADP+] A-like (Adh)

N

57

gi|66506786

Malate dehydrogenase cytoplasmic-like (MDH1)

N

57

gi|66550890

Phosphoglycerate mutase 2-like (Pglym)

N

57

gi|328779578

Lysozyme c-1

Others 4

Y

57

gi|328783193

Dehydrogenase/reductase SDR family member 11-like (SDR )

N

57

gi|328789531

Hypothetical protein LOC408666

Y

57

gi|48095525

Tubulin beta-1 chain

N

57