Figure 3

Validation of microarray transcription of selected differentially transcribed genes. A total of seven differentially transcribed genes were selected in the validation using reverse transcriptase-multiplex ligation dependent probe amplification (RT-MLPA). These genes include four genes up-regulated in CW: cellulose synthase 3 (PrCesA3), PrCesA11, cinnamic acid 4-hydroxylase (C4H) and plastocyanin-like (PCL); three genes more highly transcribed in OW: peroxidase (PER), E3 ubiquitin protein ligase (UPL1) and retinoblastoma-like protein (RBL). Developing xylem (CW and OW) sampled in autumn for the microarray experiment was used in the validation, including three biological and four technical replicates. Mean log-2 ratios (CW/OW) of the 12 replicates were calculated for the selected genes and compared with their microarray transcription results. The mean log-2 ratio values > 0 and < 0 indicate genes preferentially transcribed in CW and OW, respectively. Error bars represent the standard deviation of the mean log-2 ratio.