Figure 4

Expression of IPS1 and several upregulated transcripts under –P conditions was determined using RNA-Seq and qRT-PCR. IPS1, IPS2, RNS1, and MGD expression in roots and IPS1, SPX1, GDPD, and PAP expression in shoots under –P were also analysed using qRT-PCR at 0 and 10 d after –P treatment. Transcript expression levels were normalised using an internal control (Ubiquitin1) and plotted relative to expression on day 10. Bars represent mean ± SE from the three experiments. Fold changes based on RPKM values according to RNA-Seq are plotted on the same graph.