Table 1 Major proteomic studies performed on human urine a
Year | Title | Sample | No. of identifications | Analysis method and instrument used | Reference |
|---|---|---|---|---|---|
2004 | Identification and proteomic profiling of exosomes in human urine | exosome | 295 | 1-DE and LC–MS/MS | [25] |
2004 | Establishment of a near-standard two-dimensional human urine proteomic map | urine | 113 | 2-DE and MALDI TOF | [22] |
2005 | Exploring the hidden human urinary proteome via ligand library beads | urine | 383 | 2-DE and SELDI-TOF; LC FT-ICR | [8] |
2005 | Human urine proteome analysis by three separation approaches | urine | 226 | 1-DE and 2D LC LCQ-DECAXpplus | [24] |
2005 | Development of a high-throughput method for preparing human urine for two-dimensional electrophoresis | urine | 50 | 2-DE and 4700 TOF/TOF MS | [21] |
2006 | The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins | urine | 1543 | 1-DE and LC LTQ-Orbitrap and LC-FT | [6] |
2006 | Characterization of the human urine proteome by preparative electrophoresis in combination with 2-DE | urine | 141 | 2-DE and MALDI TOF | [20] |
2006 | Simple urinary sample preparation for proteomic analysis | urine | 339 | 2-DE and MALDI TOF | [9] |
2008 | Optimizing sample handling for urinary proteomics | urine | 735 | 1-DE and LC LTQ | [4] |
2009 | Urine proteomics for profiling of human disease using high accuracy mass spectrometry | urine | 2362 | 1-DE and LC LTQ-Orbitrap | [5] |
2009 | Large-scale proteomics and phosphoproteomics of urinary exosomes | exosome | 1132 | 1-DE and LC LTQ | [26] |
2009 | High speed two-dimensional protein separation without gel by isoelectric focusing-asymmetrical flow field flow fractionation: application to urinary proteome | urine | 245 | 2D-IEF and LC LCQ-DecaXP | [23] |
2010 | A comprehensive and non-prefractionation on the protein level approach for the human urinary proteome: touching phosphorylation in urine | urine | 1310 | 2D LC LTQ-Orbitrap | [2] |
2011 | A comprehensive map of the human urinary proteome | urine | 1823 | 1-DE and LC LTQ-Orbitrap Velos | [3] |
2012 | Analysis of the urine proteome via a combination of multi-dimensional approaches | urine | 558 | 1-DE and quadrupole ITMS | [19] |
2012 | Urine proteome of autosomal dominant polycystic kidney disease patients | urine | 1700 | IEF and LC LTQ-Orbitrap Velos | [7] |
2013 | Urinary proteomic and non-prefractionation quantitative phosphoproteomic analysis during pregnancy and non-pregnancy | urine | 2579 | 1-DE and ESI-MS/MS (LTQ-Orbitrap Velos) | this study |