Analysis of the leaf transcriptome of Musa acuminata during interaction with Mycosphaerella musicola: gene assembly, annotation and marker development

Table 2 Characteristics of polymorphic microsatellite loci isolated from M. acuminata Calcutta 4 and Cavendish Grande Naine unigene sets

Locus number	Unigene ID	SSR repeat motif	SSR locus length (bp)	Allelic Amplitude (bp)	Number of alleles	He	Ho	PIC Value
locus821	musa_c4_small_c22955	CGG	14	170-180	3	0.61	0.52	0.53
locus945	musa_c4_small_c25361	TGACA	17	325-330	3	0.62	0.57	0.55
locus1284	musa_c4_small_c7084	AG	11	270-330	6	0.77	0.65	0.74
locus1412	musa_c4_small_c8798	TGC	15	175-190	3	0.39	0.14	0.34
locus1654	musa_c4_small_rep_c12642	CCT	16	170-200	5	0.70	0.71	0.66
locus2108	musa_c4_small_rep_c2099	GA	14	190-220	7	0.81	0.92	0.79
locus2061	musa_c4_small_rep_c1992	AG	19	190-200	5	0.70	0.52	0.66
locus2108	musa_c4_small_rep_c2099	GA	14	205-230	8	0.84	0.91	0.82
locus2112	musa_c4_small_rep_c21184	GTC	18	200-205	3	0.52	0.65	0.46
locus2306	musa_c4_small_rep_c2740	TG	13	170-185	4	0.74	0.38	0.69
locus2984	musa_c4_clusters_pos_c1484_1	TCCT	13	115-130	7	0.80	0.45	0.77
locus371	musa_cav_c18572	AT	12	350-360	3	0.56	0.54	0.48
locus2028	musa_cav_rep_c22610	CTT	17	280-290	4	0.70	0.75	0.65
locus2646	musa_cav_rep_c5186	TTC	18	200-220	5	0.73	0.30	0.68

Polymorphism was evaluated across 21 M. acuminata accessions. HE, expected heterozygosity under Hardy-Weinberg expectations; HO, observed heterozygosity; PIC, Polymorphism Information Content.

ISSN: 1471-2164