Table 3 Primer sequences for qRT-PCR analysis of expression of SSR marker–derived genes associated with defense responses and BAC clone-derived LRR receptor-like serine/threonine-protein kinases
Gene / contig abbreviation | KOG Gene description / BAC annotation | Primer Sequence Forward/Reverse | Amplicon Size (bp) | PCR efficiency (%) | Efficiency SD (+/−) |
|---|---|---|---|---|---|
THAU_musa_c4_small_rep_c3564 | Pathogenesis related proteins, group 5, Thaumatin | CCGGTGGGACTAATTACAGG/ CAATTCGGATGTCAATGCAG | 165 | 99 | 0,011 |
CHIT_musa_c4_small_c3092 | Chitinase | CACCATCTCCTGCAAGCATA/ GCAGTCATTCCTCGTTGTCA | 123 | 96 | 0,008 |
M09A11_454_Ma4140M09A11 | Putative LRR receptor-like serine/threonine-protein kinase | CCAGCAACCACAACCCTAGT/ GATCTTTCAGGCCTCGTTTG | 176 | 99 | 0,009 |
B03A51_454_Mac054B03A51 | Putative LRR receptor-like serine/threonine-protein kinase | ATTCCATGGCTACGGACAAT/ TTCAGGCCTCGTTTGAACTC | 192 | 107 | 0,005 |
B03A11_454_Mac054B03A11 | Putative LRR receptor-like serine/threonine-protein kinase | CAGCAACCATAACCCCATTC/ AGGATCTTTCAGGCCTCGTT | 168 | 105 | 0,011 |
EF | Elongation Factor | AACCCCCAAATATTCCAAGG/ AGATTGGCACGAAAGGAATC | 107 | 102 | 0,013 |