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Table 2 Effects of gonadotropin treatment on timing of sperm collection and the sperm volume in fugu

From: New approach for fish breeding by chemical mutagenesis: establishment of TILLING method in fugu (Takifugu rubripes) with ENU mutagenesis

   Period after the last ENU treatment
   3 weeks 5 weeks 6 weeks
  ENU conc. (mg/kg) No. of fish from which sperm was collected/No. of fish examined (%)* Sperm volume (ml) No. of fish from which sperm was collected/No. of fish examined (%)* Sperm volume (ml) No. of fish from which sperm was collected/No. of fish examined (%)* Sperm volume (ml)
Gonadotropin treatment (2 weeks after the last ENU treatment) 70 0/4 (0) 0 2/4 (50) 5-15 3/4 (75) 4-10
Gonadotropin treatment (3 weeks after the last ENU treatment) 0 - - 2/2 (100) 4-9 1/1 (100) 6
7 - - 3/3 (100) 7.5-30 3/3 (100) 8-10
70 - - 3/3 (100) 10-17.5 3/3 (100) 5-7
120 - - 0/2 (0) 0 1/1 (100) 3
Non-gonadotropin treatment (control) 70 0/3 (0) 0   0 3/3 (100) 0.2-2
  1. (%)* reveals ratio of fish which were able to collect the ENU-mutagenized sperm to the examined fish.