Table 2 Effects of gonadotropin treatment on timing of sperm collection and the sperm volume in fugu
| Â | Â | Period after the last ENU treatment | |||||
|---|---|---|---|---|---|---|---|
| Â | Â | 3Â weeks | 5Â weeks | 6Â weeks | |||
| Â | ENU conc. (mg/kg) | No. of fish from which sperm was collected/No. of fish examined (%)* | Sperm volume (ml) | No. of fish from which sperm was collected/No. of fish examined (%)* | Sperm volume (ml) | No. of fish from which sperm was collected/No. of fish examined (%)* | Sperm volume (ml) |
Gonadotropin treatment (2Â weeks after the last ENU treatment) | 70 | 0/4 (0) | 0 | 2/4 (50) | 5-15 | 3/4 (75) | 4-10 |
Gonadotropin treatment (3Â weeks after the last ENU treatment) | 0 | - | - | 2/2 (100) | 4-9 | 1/1 (100) | 6 |
7 | - | - | 3/3 (100) | 7.5-30 | 3/3 (100) | 8-10 | |
70 | - | - | 3/3 (100) | 10-17.5 | 3/3 (100) | 5-7 | |
120 | - | - | 0/2 (0) | 0 | 1/1 (100) | 3 | |
Non-gonadotropin treatment (control) | 70 | 0/3 (0) | 0 | Â | 0 | 3/3 (100) | 0.2-2 |