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Table 3 Summary of fugu MSTN mutations detected by HRM analysis

From: New approach for fish breeding by chemical mutagenesis: establishment of TILLING method in fugu (Takifugu rubripes) with ENU mutagenesis

Family no. ENU conc. (mg/kg body weight) DNA sequence Amino acid substitution
1 7 CTGGGACTGG(A > G)CTGCGACGAG D272G
1 7 TTGCAGAAAT(A > G)CCCGCACACC Y322C
2 70 gCAACCTTTC(A > G)TGGAGGTGAA M254V
3 70 GAGACCTGGG(A > G)CTGGACTGCG G270G
3 70 CACCTGGTGA(A > G)CAAGGCCAAC N329S
4 70 AGTGTGAGTA(C > A)ATGCACTTGC Y316X
5 70 CGCACACCCA(C > T)CTGGTGAACA H326H
5 70 AGGGACCGCG(G > A)GCCCTTGCTG G338S
6 120 CATGCTCTAC(T > C)TCAACCAAGA F354L
6 120 CTCTACTTCA(A > G)CCAAGAACAG N355S
  1. (A > B): A is the nucleotide of wild-type and B is the nucleotide of the mutant. X indicates a stop codon. There were ten types of mutation in the investigated region with HRM analysis (nonsense, 1; missense, 7; silent, 2). The nonsense mutation is underlined.