New approach for fish breeding by chemical mutagenesis: establishment of TILLING method in fugu (Takifugu rubripes) with ENU mutagenesis

Table 3 Summary of fugu MSTN mutations detected by HRM analysis

Family no.	ENU conc. (mg/kg body weight)	DNA sequence	Amino acid substitution
1	7	CTGGGACTGG(A > G)CTGCGACGAG	D272G
1	7	TTGCAGAAAT(A > G)CCCGCACACC	Y322C
2	70	gCAACCTTTC(A > G)TGGAGGTGAA	M254V
3	70	GAGACCTGGG(A > G)CTGGACTGCG	G270G
3	70	CACCTGGTGA(A > G)CAAGGCCAAC	N329S
4	70	AGTGTGAGTA(C > A)ATGCACTTGC	Y316X
5	70	CGCACACCCA(C > T)CTGGTGAACA	H326H
5	70	AGGGACCGCG(G > A)GCCCTTGCTG	G338S
6	120	CATGCTCTAC(T > C)TCAACCAAGA	F354L
6	120	CTCTACTTCA(A > G)CCAAGAACAG	N355S

(A > B): A is the nucleotide of wild-type and B is the nucleotide of the mutant. X indicates a stop codon. There were ten types of mutation in the investigated region with HRM analysis (nonsense, 1; missense, 7; silent, 2). The nonsense mutation is underlined.

ISSN: 1471-2164