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Figure 6 | BMC Genomics

Figure 6

From: Transcriptome sequencing and microarray design for functional genomics in the extremophile Arabidopsis relative Thellungiella salsuginea (Eutrema salsugineum)

Figure 6

Comparison of median raw signal intensities of 15,557 genes matched between T. salsuginea and Arabidopsis arrays. T. salsuginea genes were matched with A. thaliana genes using BLASTX, with a significance cut-off of E < 10-10 for a sequence similarity search against the Arabidopsis TAIR10 database. This resulted in 15,557 genes that matched between the two microarrays. Pearson’ s correlations (p < 2.2 × 10-16) are shown between log10-transformed signal intensities obtained from T. salsuginea and Arabidopsis arrays hybridized with identical leaf RNA samples from either non-acclimated Yukon (A) or Col-0 (B), or cold acclimated Yukon (D) or Col-0 (E). The median density signal distributions of all 15,557 probes after hybridization with non-acclimated (C) or cold acclimated RNA samples (F) are also shown. The green lines indicate the density distribution of median signal intensity of Yukon samples hybridized on the array designed for Arabidopsis, while the blue lines show the density distribution of Yukon samples hybridized on the array designed for T. salsuginea. The red lines show the density distribution of Col-0 samples hybridized on the Agilent array designed for T. salsuginea and the black lines show the density distribution of Col-0 samples hybridized on the array designed for Arabidopsis.

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