Figure 5

DUF364 expression increases prodigiosin production and is controlled by a putative 5′ cis -acting regulatory RNA element. A- B. RNA-seq reads are shown mapped to the conserved hypothetical ORF (or1384; DUF364) which is divergent to pigO of the pigA-O cluster. The cumulative coverage is shown for the three replicates of the WT (A) and the hfq mutant (B). A peak was also observed in the replicates of rsmA (data not shown). The coverage is shown as raw reads aligned to the reference genome sequence in BAM strand stack view in the genome browser Artemis with the annotation displayed underneath. The colour scheme for the reads is blue for paired reads; black for single reads or reads with an unmapped pair; and green for reads that are overlaid and with the same start and end alignment positions. C. Various regions of or1384 were cloned under the T5 promoter in the vector pQE80oriT and expressed in trans in the WT to determine an effect on pigment levels. pNB46 contains the ORF preceded by the 5′UTR (−154 bp to +951 bp relative to the translation start codon); pNB47 contains the region where the peak of reads mapped in high abundance (−154 bp to +260 bp); and pNB48 contains only the ORF (+1 to +951).