Figure 10

Validation of pyro-sequencing data. A total of eight TF genes showing differential expression in our pyro-sequencing experiment were selected and their relative expression determined using qRT-PCR in two olive tissues: (A) fruit pericarp at 217 DPA and (B) fruit-AZ at 217 DPA. (C) qRT-PCR analysis of eight TF genes in olive fruit during fruit ripening. (D) qRT-PCR analysis of eight TF genes in olive AZ during abscission of mature-fruit. In the Materials and Methods section, genes and their primers are indicated. Relative expression values were normalized to the lowest expression value taken as 1. The data represent the mean values (±SEs) of duplicate experiments from three independent biological samples.