Figure 3

Phylogram of salivary protein families derived from Psorophora albipes sialotranscriptome commonly found in Culicine and Anopheline mosquito sialotranscriptomes. A, C: HHH peptide family. B, D: Mosquito basic tail family. Clustal alignment (A and B) and dendrogram (C and D) of all HHH peptide family and mosquito basic tail family, respectively, derived from the Psorophora sialotranscriptome. The symbols above the alignment indicate (*) identical sites, (:) conserved sites, and (.) less-conserved sites. The phylogram derived from the alignment of Psorophora proteins (indicate by Psor- and its contig number) with their best matches in the non-redundant database. The three first letters indicate the genus name from which each protein originates, followed by the three first letters of the species name, followed by the NCBI accession number. The numbers on the tree bifurcations indicate the percentage of bootstrap support above 75%. The scale bar at the bottom represents 10% amino acid substitution. Sequences were aligned by the ClustalW program, and the phylogram was made with the Mega package after 10,000 bootstraps with the neighbor-joining algorithm.