Table 3 Amplicon PrimerBLAST results
From: A high-throughput Sanger strategy for human mitochondrial genome sequencing
Amplicon number | Number of nuclear genome matches | Nuclear genome location | GenBank accession number | Priming region similarity (forward; reverse) | Size difference from mtDNA target (in bp) | Sequence similarity to rCRS |
|---|---|---|---|---|---|---|
1 | 1 | Chr 11 (9510471–9507925) | NT_009237.18 | 76.2%; 76.2% | 170 | < 60% |
2 | 1 | Chr 6 (53426379–53424135) | NT_025741.15 | 84.2%; 78.9% | 118 | < 60% |
3 | 1 | Chr 1 (43791–46082) | NT_004350.19 | 100%; 100% | 1 | 98.47% |
4 | 2 | Chr 1 (45818–48327) | NT_004350.19 | 95.5%; 100% | 1 | 98.57% |
Chr 5 (7704044–7701575) | NT_034772.6 | 95.5%; 90.9% | 1 | 88.53% | ||
5 | 2 | Chr 7 (6847950–6850445) | NT_033968.6 | 84.0%; 88.9% | 7 | 75.16% |
Chr 2 (10717704–10720523) | NT_022135.16 | 80.0%; 88.9% | 331 | 63.40% | ||
6 | 2 | Chr 5 (42577018–42574300) | NT_034772.6 | 95.5%; 100% | 0 | 94.02% |
Chr 5 (7699358–7696640) | NT_034772.6 | 90.5%; 90.0% | 0 | 88.97% | ||
7 | 4 | Chr 5 (2218206–2220412) | NT_034772.6 | 95.2%; 90.0% | 1 | 87.27% |
Chr 5 (2218173–2220412) | NT_034772.6 | 81.0%; 90.0% | 32 | 87.19% | ||
Chr 17 (13111672–13109937) | NT_010718.16 | 80.0%; 85.0% | 453 | < 60% | ||
Chr 7 (5831689–5829543) | NT_033968.6 | 81.0%; 75.0% | 41 | < 60% |