Table 2 PCR results of deleted or conserved genes at the GPL biosynthesis related locus from M. massiliense Type I, M. massiliense Type II, M. massiliense Type I (rough colony morphology), and M. abscessus (smooth and rough colony morphology), strains

Deleted genes in M. massiliense Type II strain

atf1

21/21 (100)

0/21 (0)

12/12 (100)

11/11 (100)

11/11 (100)

atf2

21/21 (100)

0/21 (0)

12/12 (100)

11/11 (100)

11/11 (100)

fmt

21/21 (100)

0/21 (0)

12/12 (100)

11/11 (100)

11/11 (100)

gtf1

21/21 (100)

0/21 (0)

12/12 (100)

11/11 (100)

11/11 (100)

gtf2

21/21 (100)

0/21 (0)

12/12 (100)

11/11 (100)

11/11 (100)

mps1

21/21 (100)

0/21 (0)

12/12 (100)

11/11 (100)

11/11 (100)

mps2

21/21 (100)

0/21 (0)

12/12 (100)

11/11 (100)

11/11 (100)

rmt2

21/21 (100)

0/21 (0)

12/12 (100)

11/11 (100)

11/11 (100)

rmt3

21/21 (100)

0/21 (0)

12/12 (100)

11/11 (100)

11/11 (100)

rmt4

21/21 (100)

0/21 (0)

12/12 (100)

11/11 (100)

11/11 (100)

Conserved genes

gap

21/21 (100)

21/21 (100)

12/12 (100)

11/11 (100)

11/11 (100)

rmlB

21/21 (100)

21/21 (100)

12/12 (100)

11/11 (100)

11/11 (100)