Figure 5

Comparison of mRNA gene expression between the microarray and reverse transcription-quantitative PCR (RT-qPCR). Genes that have shown a significant difference in expression between Staphylococcus aureus infected cells and uninfected control cells in RT-qPCR analysis are denoted with p ≤ 0.05 and non-significant genes in RT-qPCR are denoted with p > 0.05. Genes that have shown a divergent expression pattern in the RT-qPCR analysis compared to the microarray experiment are denoted with ≠. Bcl2-antagonist of cell death (BAD), caspase 1 (CASP1), chemokine c-c motif ligand 5 (CCL5), chemokine c-c motif receptor 5 (CCR5), v-fos fbj murine osteosarcoma viral oncogene homolog (FOS), intercellular adhesion molecule-1 (ICAM1), interferon beta (IFNb), interferon regulatory factor 1 (IRF1), mitogen-activated protein kinase 14, p38 (MAPK14), p21/cdc42/rac1-activated kinase 1 (PAK1), remodeling and spacing factor 1 (RSF1), Toll-like receptor 8 (TLR8) and tumor necrosis factor alpha (TNFa). Peptidylprolyl isomerase A (PPIA) was used as a reference gene.