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Figure 3 | BMC Genomics

Figure 3

From: Male infertility and copy number variants (CNVs) in the dog: a two-pronged approach using Computer Assisted Sperm Analysis (CASA) and Fluorescent In Situ Hybridization (FISH)

Figure 3

FISH experiments on lymphocytes. A. Co-hybridizations of BACs CH82-321J09, CH82-324I01, and CH82-509B23 (Cy-3 labeled, in red) with single-copy control BACs CH82-297B18 and CH82-53P08 (Cy-5 labeled, in green) on lymphocytes from 6 samples. Bigger sizes of hybridization signals for BAC CH82-321 J09 and CH82-509B23 compared with single-copy control BACs CH82-297B18 and CH82-53P08 reveal duplications in those genomic regions in all the analyzed samples except for sample #12. Sample #11 shows bigger hybridization signals for probe CH82-324I01 compared with other samples and single-copy control probe, suggesting a clear and isolated duplication pattern. B. same co-hybridizations on sperm cells from the 5 out of the 6 samples shown in part A.

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