Figure 3
From: Ultra-deep mutant spectrum profiling: improving sequencing accuracy using overlapping read pairs
Q-score distributions for all matching (blue), mismatched (red) and erroneous matching (green) overlapping read pairs in the BCV control sample. Note, the Q-score of 2 is a ‘read segment control indictor’ in the FASTQ format that tags specific final portion of the read as unreliable and unfit for downstream analyses [41]. That Q=2 reads comprised a disproportionally large fraction of mismatched read pairs (red) is consistent with the fact that mismatched ORPs result from error during sequencing.