Figure 6

Quantitative RT-PCR validation of tag-mapped genes associated with cotton anther development. TPM, Transcription per million mapped reads. F-1 and S-1: meiosis stage of WT and GMS mutant anthers; F-2 and S-2: tetrad stage of WT and GMS mutant anthers; F-3 and S-3: uninucleate microspore stage of WT and GMS mutant anthers. Relative expression levels were calculated using 18S RNA as an internal control.