Figure 2

Visual representation of position-based and feature-based domain hotspots. The structure of the Ras-like protein domain (cd00882) of the human protein Cdc42 (PDB: 1CEE_A) is shown in purple (left). The sequence logo (generated using the WebLogo software [62]) represents a subset of the Ras-like protein domain from positions 1-152 (right). The functional feature residues corresponding to the GTP/Mg++ binding site at domain positions 5-11, 54, 110, 111, 113, and 141-143 are highlighted in orange on the structure (left) and are represented as orange boxes below each domain position in the sequence logo (right). Each of these functional feature protein domain positions will have a different position-based DS-Score (estimated based on number of mutations at each domain position) but equal feature-based DS-Score (estimated based on the maximum number of mutations found in any of the binding sites).