Figure 7

Somatic mutation in CDKN2A predicted to be deleterious by MutationAssessor. MutationAssessor predicted the somatic D125N mutation in the canonical tumor suppressor CDKN2A to be deleterious, due to its conservation in mammalian orthologs (Subfamily 1). Other tools used a wider array of homologs, including fish orthologs, where the residue is in fact N, and so classified the mutation to be benign. Using PyMOL (Version 1.2r3pre, Schrödinger, LLC.) the protein structure (PDB id: 1BI7) illustrates CDKN2A (wheat color) in complex with CDK6 (blue). The majority of residues in CDKN2A are known to be implicated in cancer based on UniProt (http://www.uniprot.org) annotation (green). D125 is shown in orange.