Figure 3

Phylogenic constructions of Sphingobium spp. Sphingobium baderi LL03, S. lactosutens DS20, S. chinhatense IP26, S. quisquilarium P25, S. ummariense RL3, Sphingobium sp. HDIPO4, S. indicum B90A, S. japonicum UT26S and Sphingobium sp. SYK6. (A) 16S rRNA gene sequence-based phylogeny was performed with the neighbor joining method using TreeconW 1.3b (bootstrap value = 1000) (B) The amino acid sequences of 28 single copy genes were concatenated for all ten genomes and phylogeny performed with the neighbor joining method using TreeconW 1.3b (bootstrap value =1000) (C) &(D) Whole genome based tetranucleotide correlation and ANI based pairwise comparisions between the genomes was carried out for all the genomes. A pearson correlation matrix was constructed on the basis of their tetranucleotide correlation and ANI values, followed by hierarchical clustering on the resultant matrix using MEV4.9.0, and visualized on MEGA4.