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Table 2 Mutations identified by whole-exome sequencing

From: Simulation and estimation of gene number in a biological pathway using almost complete saturation mutagenesis screening of haploid mouse cells

    (SnpEff§) High Moderate Low Modifier
Clone name Responsible genes* Total no. of mutations Nonsense mutations Splice site alterations Frameshift mutations Loss of start codon Nonsynonymous mutations etc.|| Synonymous mutations Others¶  
B3-1 Pigt 220 6 5 0 0 115 39 1 54
B5-1 Dpm1 297 6 3 0 1 128 60 2 97
B7-2 Pigo 218 5 1 0 0 103 38 1 70
B102 Pigb 10 0 0 1 0 4 0 0 5
B201 Pigk 41 1 1 0 0 14 7 0 18
B502 Dpm1, Pigv 511 13 12 1 1 241 110 5 128
B1001 Pigv 306 8 2 0 0 127 55 1 113
B1002 Pigs 330 10 2 1 0 152 63 0 102
B1007 Gpaa1 23 1 1 0 0 9 2 1 9
F-43 Pigo 375 12 4 2 0 153 83 2 119
Average 233.1 10 104.6 47 71.5
  1. *Genes whose mutations are responsible for the host cell phenotypes.
  2. †All exons are deleted (Additional file 7: Figure S4).
  3. ‡Undetectable because of an exome design defect (Additional file 2: Figure S2).
  4. §Also explained in Additional file 6: Table S12.
  5. ||The remainder: one codon insertion in clone B102.
  6. ¶Others: start gained; synonymous stop.