Figure 1
Increased concentration of CCL2, TNFα, and IFN-α in lung homogenates of A/Hong Kong/213/03 H5N1 virus infected DBA/2J mice. (A) DBA/2J (D2) and C57BL/6J (B6) were inoculated with 104 EID50 of HK213 virus (+) or mock-inoculated (-) in 30 μl PBS. Forty-eight hours post inoculation the lungs of the inoculated animals were collected, homogenized in sterile PBS, and stored at -80°C. The concentration of CCL2, TNFα, and IFN-α in these homogenates was quantified by ELISA. The average cytokine concentration (pg/ml ± standard error of the mean) of CCL2, TNFα and IFN-α is shown for mock-infected (n =4, single experiment), C57BL/6 (n =13 from 4 different experiments), and DBA/2J (n =15 from four different experiments). *** is P <0.0001. (B) Significant association between CCL2, TNF-α, and IFN-α concentration and lung virus titer 48 hours post inoculation with 104 EID50 of HK213 virus in B6, D2 and BXD recombinant inbred mouse strains (P < 0.0001 for all three cytokines).