Figure 4

Protein expression changes in pancreatic islets of RasGrf1 KO mice. Western immunoblot and Immunohystochemistry assays confirming and validating several transcriptional alterations previously detected by means of microarray analysis in pancreatic islets from RasGrf1 KO mice. (A) Representative ECL exposures of WB assays performed in protein extracts of WT and KO pancreatic islets, using specific antibodies against IAPP, PDFR, FGFR, TXNL or PTTG1. Unchanged Ras and beta-tubulin signals are shown as internal loading controls. (B) Representative immunohystochemical staining of WT and KO pancreatic sections showing increased expression levels of the GRIA2 and PHTF1 proteins in pancreatic islets of RasGrf1 KO mice (islets outlined in yellow). (C) Normalized PTTG1 to Beta-tubulin signal ratios in Western blots of WT and KO islets quantitated using the image-J software program. For comparison purposes, this ratio was set to 1 in WT samples.