Figure 6

The DNA and the amino acid sequences alignments of a partial sequence of a cytochrome P450 CYP6AX1 ortholog between grain aphid and pea aphid. The DNA and amino acid sequences alignments of a cytochorome P450 CYP6AX1 orthologs in grain aphid (aphid_c9724) and pea aphid (ACYPI002699-RA). The Ka/Ks value of this ortholog between the two species is 3.41(p-value of 0.049). The loci shadowed with blue color are synonymous substitutions without amino acid changes, whereas the loci shadowed with pink color are nonsynonymous substitutions with amino acids changes. The amino acid changes without shadowed color indicated ‘not determined’ due to a presence of an ‘N’ in sequencing. The substitution marked with *** is predicted as a locus under positive selection based on KaKs calculation with the sliding window length (underlined) and step length of 57 bp and 6 bp, respectively. (KaKs_Calculator 2.0, Ka/Ks: 1.266).