Phosphorylation modifications of serpins in the developing grains of Yanyou 361. A: The part sequence alignment of serpins identified in the current study, and the red box indicated the phosphorylated sites. B: Three-dimensional structure map of serpin3 (C0LF32) and serpin-N3.2 (H9AXB3) predicted by Phyre. The phosphorylated sites Thr7, Ser131, Ser147 and Ser165 are marked with red color, and a part of domains are marked. C: The representative MS/MS spectra of the common phosphopeptide _ATTLAT(ph)DVR_ present in four serpins.